Although the clinical importance of prostate calculi has been debatable, it is a disease that can cause a plethora of symptoms and signs – sometimes in disguise. Clean intermittent catheter (CIC) is the gold standard method for bladder rehabilitation / training in patients with floppy / atonic bladders (in both males and females). The aim of this study was to compare the incidence of prostate calculi and related pathologies between patients using CIC and not using CIC. A total of 314 neurogenic bladder patients who were followed up and treated were included in this study. There were two groups: patients not-using CIC (Group 1, n:154) and patients using CIC (Group 2, n:160). The parameters were: presence of prostate calculi, the number of CIC used per day, plasma uric acid levels, urine parameters, mean-stone-density (MSD) and calculi sizes, which were retrospectively collected from patient records. In this study, no significant difference was observed between the parameters such as age, uric acid level, MSD, urine parameters, and other electrolyte levels. However, the incidence of prostate calculi in Group 1 was 23.4% and in group 2 it was 37.5% (p=0.007). As a result, it was shown that the incidence of prostate calculi increased in patients using CIC. This may lead to increasing lower urinary tract symptoms, dysuria, recurrent urinary tract infections, etc. In other studies it has been shown that prostatic calculi do not influence PSA levels.